NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM4407508 Query DataSets for GSM4407508
Status Public on Mar 12, 2020
Title 0.5 M Exo 2
Sample type other
 
Source name CTL culture sup.
Organism Mus musculus
Characteristics treatment: 0.5 M NaCl eluted Exo
Treatment protocol Murine CTL culture supernatant was subjected to DEAE column chromatograpy after concentration of deproteinization of 750 kDa MWCO ultrafiltration.
Growth protocol Murine CTL was cultured for 7days, and culture supernatant was collected.
Extracted molecule other
Extraction protocol Without extraction process, 2 x 10^9 particles/mL EVs were labeled and subjected lectin maicroarray.
Label Cy3
Label protocol Hydrophobic fraction was labeled with Cy3 NHS ester (GE Healthcare).
 
Hybridization protocol Labeled EVs were incubated for o/n on Rexxam (96) lectin array chip.
Scan protocol Fluorescence images were acquired using an evanescent field-activated fluorescence scanner (GlycoStationTM reader 1200; GP BioSciences).
Description Evanescent-wave fluorescence excitation method
Data processing The fluorescence signal of each spot was quantified using Array Pro Analyzer version 4.5 (Media Cybernetics, Bethesda), and the background value was subtracted. The background value was obtained from the area without lectin immobilization.
 
Submission date Mar 11, 2020
Last update date Mar 12, 2020
Contact name Naohiro Seo
E-mail(s) seo-naohiro@clin.medic.mie-u.ac.jp
Organization name Mie University Graduate School of Medicine
Department Immuno-Gene Therpy
Street address 2-174 Edobashi
City Tsu
State/province Mie
ZIP/Postal code 514-8507
Country Japan
 
Platform ID GPL28263
Series (1)
GSE146818 Lectin microarray analysis of murine CTL EVs separated by ion exchange method

Data table header descriptions
ID_REF
VALUE The lectin signals of triplicate spots were averaged and normalized to the mean value of 96 lectins immobilized on the array.
0.5 M Exo 2 raw data

Data table
ID_REF VALUE 0.5 M Exo 2 raw data
1 119.2579311 10864
2 85.17325918 7759
3 259.6801932 23656
4 42.09813751 3835
5 37.79501628 3443
6 80.97991145 7377
7 173.5079952 15806
8 141.6407479 12903
9 135.1750887 12314
10 152.0033664 13847
11 196.1872004 17872
12 134.4176516 12245
13 95.01994219 8656
14 43.20684987 3936
15 104.0103919 9475
16 121.1899447 11040
17 22.18522449 2021
18 180.3359069 16428
19 29.72666398 2708
20 300.6037543 27384

Total number of rows: 96

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap