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Sample GSM4407509 Query DataSets for GSM4407509
Status Public on Mar 12, 2020
Title 0.5 M Exo 3
Sample type other
 
Source name CTL culture sup.
Organism Mus musculus
Characteristics treatment: 0.5 M NaCl eluted Exo
Treatment protocol Murine CTL culture supernatant was subjected to DEAE column chromatograpy after concentration of deproteinization of 750 kDa MWCO ultrafiltration.
Growth protocol Murine CTL was cultured for 7days, and culture supernatant was collected.
Extracted molecule other
Extraction protocol Without extraction process, 2 x 10^9 particles/mL EVs were labeled and subjected lectin maicroarray.
Label Cy3
Label protocol Hydrophobic fraction was labeled with Cy3 NHS ester (GE Healthcare).
 
Hybridization protocol Labeled EVs were incubated for o/n on Rexxam (96) lectin array chip.
Scan protocol Fluorescence images were acquired using an evanescent field-activated fluorescence scanner (GlycoStationTM reader 1200; GP BioSciences).
Description Evanescent-wave fluorescence excitation method
Data processing The fluorescence signal of each spot was quantified using Array Pro Analyzer version 4.5 (Media Cybernetics, Bethesda), and the background value was subtracted. The background value was obtained from the area without lectin immobilization.
 
Submission date Mar 11, 2020
Last update date Mar 12, 2020
Contact name Naohiro Seo
E-mail(s) seo-naohiro@clin.medic.mie-u.ac.jp
Organization name Mie University Graduate School of Medicine
Department Immuno-Gene Therpy
Street address 2-174 Edobashi
City Tsu
State/province Mie
ZIP/Postal code 514-8507
Country Japan
 
Platform ID GPL28263
Series (1)
GSE146818 Lectin microarray analysis of murine CTL EVs separated by ion exchange method

Data table header descriptions
ID_REF
VALUE The lectin signals of triplicate spots were averaged and normalized to the mean value of 96 lectins immobilized on the array.
0.5 M Exo 3 raw data

Data table
ID_REF VALUE 0.5 M Exo 3 raw data
1 123.627759 11124
2 73.26089423 6592
3 265.6485368 23903
4 43.4208607 3907
5 38.4753058 3462
6 79.19556011 7126
7 164.2591045 14780
8 144.4657712 12999
9 137.2308134 12348
10 146.7996286 13209
11 227.9845243 20514
12 131.4628516 11829
13 95.03244942 8551
14 40.88695841 3679
15 104.3345381 9388
16 116.5706189 10489
17 21.84935043 1966
18 169.5380676 15255
19 28.05074288 2524
20 296.3665453 26667

Total number of rows: 96

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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