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Sample GSM4407510 Query DataSets for GSM4407510
Status Public on Mar 12, 2020
Title UC Exo 1
Sample type other
 
Source name CTL culture sup.
Organism Mus musculus
Characteristics treatment: UC prepared Exo
Treatment protocol Murine CTL culture supernatant was subjected to DEAE column chromatograpy after concentration of deproteinization of 750 kDa MWCO ultrafiltration.
Growth protocol Murine CTL was cultured for 7days, and culture supernatant was collected.
Extracted molecule other
Extraction protocol Without extraction process, 2 x 10^9 particles/mL EVs were labeled and subjected lectin maicroarray.
Label Cy3
Label protocol Hydrophobic fraction was labeled with Cy3 NHS ester (GE Healthcare).
 
Hybridization protocol Labeled EVs were incubated for o/n on Rexxam (96) lectin array chip.
Scan protocol Fluorescence images were acquired using an evanescent field-activated fluorescence scanner (GlycoStationTM reader 1200; GP BioSciences).
Description Evanescent-wave fluorescence excitation method
Data processing The fluorescence signal of each spot was quantified using Array Pro Analyzer version 4.5 (Media Cybernetics, Bethesda), and the background value was subtracted. The background value was obtained from the area without lectin immobilization.
 
Submission date Mar 11, 2020
Last update date Mar 12, 2020
Contact name Naohiro Seo
E-mail(s) seo-naohiro@clin.medic.mie-u.ac.jp
Organization name Mie University Graduate School of Medicine
Department Immuno-Gene Therpy
Street address 2-174 Edobashi
City Tsu
State/province Mie
ZIP/Postal code 514-8507
Country Japan
 
Platform ID GPL28263
Series (1)
GSE146818 Lectin microarray analysis of murine CTL EVs separated by ion exchange method

Data table header descriptions
ID_REF
VALUE The lectin signals of triplicate spots were averaged and normalized to the mean value of 96 lectins immobilized on the array.
UC Exo 1 raw data

Data table
ID_REF VALUE UC Exo 1 raw data
1 126.4120059 4875
2 76.91036092 2966
3 243.3074567 9383
4 24.91937183 961
5 11.82438455 456
6 74.68032349 2880
7 158.9549941 6130
8 127.9937766 4936
9 146.4304815 5647
10 151.4351004 5840
11 222.7185064 8589
12 139.0143105 5361
13 113.0577119 4360
14 41.90395929 1616
15 180.3737258 6956
16 163.337277 6299
17 14.88420336 574
18 219.5290342 8466
19 14.23593666 549
20 311.1680145 12000

Total number of rows: 96

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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