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Sample GSM4407512 Query DataSets for GSM4407512
Status Public on Mar 12, 2020
Title UC Exo 3
Sample type other
 
Source name CTL culture sup.
Organism Mus musculus
Characteristics treatment: UC prepared Exo
Treatment protocol Murine CTL culture supernatant was subjected to DEAE column chromatograpy after concentration of deproteinization of 750 kDa MWCO ultrafiltration.
Growth protocol Murine CTL was cultured for 7days, and culture supernatant was collected.
Extracted molecule other
Extraction protocol Without extraction process, 2 x 10^9 particles/mL EVs were labeled and subjected lectin maicroarray.
Label Cy3
Label protocol Hydrophobic fraction was labeled with Cy3 NHS ester (GE Healthcare).
 
Hybridization protocol Labeled EVs were incubated for o/n on Rexxam (96) lectin array chip.
Scan protocol Fluorescence images were acquired using an evanescent field-activated fluorescence scanner (GlycoStationTM reader 1200; GP BioSciences).
Description Evanescent-wave fluorescence excitation method
Data processing The fluorescence signal of each spot was quantified using Array Pro Analyzer version 4.5 (Media Cybernetics, Bethesda), and the background value was subtracted. The background value was obtained from the area without lectin immobilization.
 
Submission date Mar 11, 2020
Last update date Mar 12, 2020
Contact name Naohiro Seo
E-mail(s) seo-naohiro@clin.medic.mie-u.ac.jp
Organization name Mie University Graduate School of Medicine
Department Immuno-Gene Therpy
Street address 2-174 Edobashi
City Tsu
State/province Mie
ZIP/Postal code 514-8507
Country Japan
 
Platform ID GPL28263
Series (1)
GSE146818 Lectin microarray analysis of murine CTL EVs separated by ion exchange method

Data table header descriptions
ID_REF
VALUE The lectin signals of triplicate spots were averaged and normalized to the mean value of 96 lectins immobilized on the array.
UC Exo 3 raw data

Data table
ID_REF VALUE UC Exo 3 raw data
1 140.3913228 5501
2 86.38877074 3385
3 273.3560187 10711
4 24.8830285 975
5 12.42875372 487
6 84.42364951 3308
7 171.6035729 6724
8 144.0153126 5643
9 128.0901744 5019
10 136.8438962 5362
11 218.2815823 8553
12 137.0480647 5370
13 114.8192259 4499
14 39.60867716 1552
15 190.693322 7472
16 164.4831986 6445
17 13.70480647 537
18 197.6095279 7743
19 11.22926414 440
20 299.1578052 11722

Total number of rows: 96

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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