|
Status |
Public on Jun 09, 2023 |
Title |
iPSC_CB RNA-seq |
Sample type |
SRA |
|
|
Source name |
iPSC
|
Organism |
Homo sapiens |
Characteristics |
tissue: iPSC differentiation stage: none genotype: Wild type iPSCs line
|
Extracted molecule |
total RNA |
Extraction protocol |
Cultured samples were directly lysis into RLT plus buffer and total RNA were extracted using RNeasy plus Micro Kit (Qiagen) Cells were directly lysis into RLT plus buffer and total RNA were extracted using RNeasy plus Micro Kit (Qiagen) Cultured samples were directly lysis into RLT plus buffer and total RNA were extracted using RNeasy plus Micro Kit (Qiagen) TruSeq Stranded Total RNA kit Gold (Illumina) or Whole transcriptome Ovation Solo RNA seq Library Preparation Kit (TECAN, NuGEN) was used for the library preparation of all samples
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
illumina bcl2fastq (v2.19.1.403) used for basecalling and demultiplexing Tophat (v2.1.1) used for alignment HTSeq-count (v0.6.1) used for transcriptome abundance measurement DESeq2(v1.20.0) used for differential gene expression analyses Genome_build: hg38 Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each sample
|
|
|
Submission date |
Mar 25, 2020 |
Last update date |
Jun 09, 2023 |
Contact name |
Hyun-Woo Jeong |
E-mail(s) |
zionjeong@gmail.com
|
Phone |
+4917631115236
|
Organization name |
Max-Planck-Institute for Molecular Biomedicine
|
Department |
Dept. Tissue Morphogenesis
|
Lab |
Adams
|
Street address |
Roentgenstr.20
|
City |
Muenster |
State/province |
NRW |
ZIP/Postal code |
48149 |
Country |
Germany |
|
|
Platform ID |
GPL18573 |
Series (2) |
GSE147513 |
RNA-Seq analysis of human skeletal muscle organoids |
GSE147514 |
Transcriptome profiling of human skeletal muscle organoids |
|
Relations |
BioSample |
SAMN14446520 |
SRA |
SRX7992117 |