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Sample GSM444378 Query DataSets for GSM444378
Status Public on Oct 30, 2018
Title E11.5 Cont heart, Biological Rep 6
Sample type RNA
 
Source name E11.5 heart, Nkx2-5+/IRES-CRE
Organism Mus musculus
Characteristics organ: heart
tissue: cardiac
developmental stage: E11.5
genotype: Nkx2-5+/IRES-CRE
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from individual hearts using Trizol according to the manufacter's instructions (Invitrogen).
Label biotin
Label protocol 100ng of total RNA was labeled and hybridized to Affymetrix mouse GeneChip® Mouse Gene 1.0 ST Array as per manufacturer's instructions.
 
Hybridization protocol As per standard Affymetrix protocol for the Mouse Gene 1.0 ST Array.
Scan protocol Arrays were scanned using the Affymetrix Scanner 3000.
Description Gene expression data from a single heart from Nkx2-5+/IRES-CRE mouse (Sibling control)
Data processing Log2 transformation and Robust Multichip Average (RMA) normalization method was applied using Expression Console software using the following parameters: affymetrix-algorithm-param-apt-opt-analysis-spec=rma-bg,quant-norm, sketch=-1, bioc=true, lowprecision=false, usepm=true, subsetmd5=79d5db3133f5267f6c61d65ecbaaf825,pm-only,med-polish, expon=false, attenuate=true, l=0.005, h=-1,expr, genotype=false, strand=false.
 
Submission date Aug 25, 2009
Last update date Oct 30, 2018
Contact name Timothy J Mohun
E-mail(s) tmohun@nimr.mrc.ac.uk
Phone +44 (0)208 816 2522
Fax +44 (0)208 816 2009
Organization name MRC National Institute for Medical Research
Department Developmental Biology Division
Lab Mohun Lab
Street address The Ridgeway, Mill Hill,
City London
ZIP/Postal code NW7 1AA,
Country United Kingdom
 
Platform ID GPL6246
Series (1)
GSE17807 Genome-wide binding of Nkx2-5

Data table header descriptions
ID_REF
VALUE log2 transformed, RMA normalized signal intensity

Data table
ID_REF VALUE
10338001 4.42
10338002 6.07
10338003 4.77
10338004 4.94
10338005 1.62
10338006 1.81
10338007 2.14
10338008 2.95
10338009 8.50
10338010 1.69
10338011 5.34
10338012 1.71
10338013 1.50
10338014 1.54
10338015 1.57
10338016 7.58
10338017 5.31
10338018 6.58
10338019 4.69
10338020 8.43

Total number of rows: 35557

Table truncated, full table size 487 Kbytes.




Supplementary file Size Download File type/resource
GSM444378.CEL.gz 3.7 Mb (ftp)(http) CEL
GSM444378.chp.gz 270.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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