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Sample GSM444700 Query DataSets for GSM444700
Status Public on Dec 31, 2009
Title Global expression profile of HeLa S3 cells, replicate #1
Sample type RNA
 
Source name HeLa S3 cells
Organism Homo sapiens
Characteristics gender: female
cell type: cultured HeLa S3 cells
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNeasy (Qiagen Inc., Valencia, CA) according to the manufacturer’s protocol. The RNA was then amplified using the Illumina RNA amplification system (Ambion, Austin, TX) and 750 ng was used for hybridization.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA (Whole-Genome Gene Expression with IntelliHyb Seal System Manual, 2006, Illumina).
 
Hybridization protocol Hybridization to Illumina Sentrix Expression BeadChip, staining and washing was carried out according to the manufacturer’s protocol (Illumina, Wallingford).
Scan protocol Scanning procedures were carried out as described by Illumina, Wallingford.
Description Expression profile from HELA S3 cells.
Data processing Data was analyzed using the Illumina Bead Studio software suite. The average signal for each reporter was cubic-spline normalized between chips to derive a standardized expression value. Expressed genes were identified by signal values higher than internal spike-in controls for expression (Smax>3000).
 
Submission date Aug 26, 2009
Last update date Dec 31, 2009
Contact name Stephen A Krawetz
E-mail(s) steve@compbio.med.wayne.edu
Organization name Wayne State University
Street address 275 East Hancock, CS Mott Center
City Detroit
State/province MI
ZIP/Postal code 48207
Country USA
 
Platform ID GPL6104
Series (1)
GSE13774 Nuclear scaffold and matrix attachment of HeLa chromatin

Data table header descriptions
ID_REF
VALUE Cubic Spline Normalized Signal

Data table
ID_REF VALUE
ILMN_1762337 122.6
ILMN_1736007 156.8
ILMN_1779670 186.2
ILMN_1806310 183.3
ILMN_1671474 140.7
ILMN_1772582 175.2
ILMN_1653355 144.7
ILMN_1717783 161.1
ILMN_1787689 207.6
ILMN_1731507 124.7
ILMN_1814316 177.8
ILMN_1745607 2474.8
ILMN_1757454 198.5
ILMN_1735045 233.7
ILMN_1680754 259.4
ILMN_1659452 148.6
ILMN_1767388 462.8
ILMN_1673870 165
ILMN_1675204 165.1
ILMN_1755321 1391.2

Total number of rows: 22184

Table truncated, full table size 414 Kbytes.




Supplementary file Size Download File type/resource
GSM444700.txt.gz 186.4 Kb (ftp)(http) TXT
Processed data included within Sample table

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