GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM4451503 Query DataSets for GSM4451503
Status Public on Nov 16, 2020
Title m_15733_wt
Sample type SRA
Source name Germinal center B cells
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: Germinal center B cells
cell population: AID-GFP+ cells
genotype: wildtype
treatment: Immunized, on Day 9
Treatment protocol Immunized with NP(18)OVA + alum, splenocytes isolated on Day 9
Growth protocol AID-GFP reporter mice
Extracted molecule total RNA
Extraction protocol GFP+ cells were sorted for total RNA
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Data processing Illumina Casava1.7 software used for basecalling.
Reads were aligned to the reference mouse genome version GRCm38 (gene annotation Ensembl v98) using STAR version 2.5
Gene expressions are obtained with featureCounts v1.5
DESeq2 version 1.14.1 was used to normalize raw counts and compute differential expression between wt and ko conditions. 
Genome_build: GRCm38
Supplementary_files_format_and_content: normalized count matrix from DESeq2
Submission date Apr 02, 2020
Last update date Nov 16, 2020
Contact name André Veillette
Phone 5149875605
Organization name IRCM
Department Molecular Oncology
Lab Veillette
Street address 110 Des Pins Ouest Rm1065
City Montreal
State/province QC
ZIP/Postal code H2W 1R7
Country Canada
Platform ID GPL17021
Series (1)
GSE147990 SFR KO mice germinal center B cell RNA sequencing
BioSample SAMN14526326
SRA SRX8047544

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap