|
| Status |
Public on Sep 09, 2009 |
| Title |
CD49a_03-04-04 |
| Sample type |
RNA |
| |
|
| Source name |
normal prostate
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: prostate
cell type: CD49a+ MACS sorted cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Prostate tissue was MACS sorted for positive cells. Total RNA was extracted from the cell lysate using Rneasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
|
| Scan protocol |
Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
|
| Description |
Prostate stromal fibromuscular cells.
|
| Data processing |
Raw CEL intensity data were RMA normalized using R/Bioconductor
|
| |
|
| Submission date |
Sep 01, 2009 |
| Last update date |
Sep 08, 2009 |
| Contact name |
Ricardo Z.N. Vêncio |
| Organization name |
Universidade de São Paulo
|
| Department |
Computing and Mathematics Department
|
| Lab |
http://labpib.fmrp.usp.br
|
| Street address |
Av. Bandeirantes, 3900
|
| City |
Ribeirao Preto |
| State/province |
SP |
| ZIP/Postal code |
14049-900 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE17906 |
Gene expression down-regulation in prostate tumor-associated stromal cells involves organ-specific genes |
|
| Relations |
| Reanalyzed by |
GSE20125 |
