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Sample GSM447176 Query DataSets for GSM447176
Status Public on Sep 09, 2009
Title CP_Str_08-028_CD90posi
Sample type RNA
 
Source name cancer prostate
Organism Homo sapiens
Characteristics tissue: prostate
cell type: CD90+ MACS sorted cells
gleason: 3+4
tumor stage: T2c
tumor volume: 2.5 cc
Extracted molecule total RNA
Extraction protocol Prostate tissue was MACS sorted for positive cells. Total RNA was extracted from the cell lysate using Rneasy kit (Qiagen).
Label biotin
Label protocol Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
 
Hybridization protocol Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
Scan protocol Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
Description Prostate tumor-associated stromal cells.
Data processing Raw CEL intensity data were RMA normalized using R/Bioconductor
 
Submission date Sep 01, 2009
Last update date Sep 08, 2009
Contact name Ricardo Z.N. Vêncio
Organization name Universidade de São Paulo
Department Computing and Mathematics Department
Lab http://labpib.fmrp.usp.br
Street address Av. Bandeirantes, 3900
City Ribeirao Preto
State/province SP
ZIP/Postal code 14049-900
Country Brazil
 
Platform ID GPL570
Series (1)
GSE17906 Gene expression down-regulation in prostate tumor-associated stromal cells involves organ-specific genes
Relations
Reanalyzed by GSE20125

Data table header descriptions
ID_REF
VALUE RMA-normalized log2 signal

Data table
ID_REF VALUE
1007_s_at 9.06302548103696
1053_at 6.66586395154413
117_at 9.74626085953136
121_at 7.78945872710327
1255_g_at 3.23756352466452
1294_at 6.87663411687681
1316_at 5.63757352822247
1320_at 5.42516454902917
1405_i_at 5.45791577381656
1431_at 3.80317181712756
1438_at 6.15534249428881
1487_at 8.5393902870056
1494_f_at 5.73568091408
1552256_a_at 6.84542917829684
1552257_a_at 7.80611808073974
1552258_at 4.71204199260295
1552261_at 4.87831647342526
1552263_at 5.31258364962969
1552264_a_at 6.6384535045929
1552266_at 4.62398400896016

Total number of rows: 54675

Table truncated, full table size 1478 Kbytes.




Supplementary file Size Download File type/resource
GSM447176.CEL.gz 5.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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