|
Status |
Public on Sep 09, 2009 |
Title |
08-032_CP_strom_CD90posi |
Sample type |
RNA |
|
|
Source name |
cancer prostate
|
Organism |
Homo sapiens |
Characteristics |
tissue: prostate cell type: CD90+ MACS sorted cells gleason: 4+4 tumor stage: T3b tumor volume: 2.7 cc
|
Extracted molecule |
total RNA |
Extraction protocol |
Prostate tissue was MACS sorted for positive cells. Total RNA was extracted from the cell lysate using Rneasy kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
|
|
|
Hybridization protocol |
Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
|
Scan protocol |
Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
|
Description |
Prostate tumor-associated stromal cells.
|
Data processing |
Raw CEL intensity data were RMA normalized using R/Bioconductor
|
|
|
Submission date |
Sep 01, 2009 |
Last update date |
Sep 08, 2009 |
Contact name |
Ricardo Z.N. Vêncio |
Organization name |
Universidade de São Paulo
|
Department |
Computing and Mathematics Department
|
Lab |
http://labpib.fmrp.usp.br
|
Street address |
Av. Bandeirantes, 3900
|
City |
Ribeirao Preto |
State/province |
SP |
ZIP/Postal code |
14049-900 |
Country |
Brazil |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE17906 |
Gene expression down-regulation in prostate tumor-associated stromal cells involves organ-specific genes |
|
Relations |
Reanalyzed by |
GSE20125 |