NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM447178 Query DataSets for GSM447178
Status Public on Sep 09, 2009
Title 08-032_CP_strom_CD90posi
Sample type RNA
 
Source name cancer prostate
Organism Homo sapiens
Characteristics tissue: prostate
cell type: CD90+ MACS sorted cells
gleason: 4+4
tumor stage: T3b
tumor volume: 2.7 cc
Extracted molecule total RNA
Extraction protocol Prostate tissue was MACS sorted for positive cells. Total RNA was extracted from the cell lysate using Rneasy kit (Qiagen).
Label biotin
Label protocol Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
 
Hybridization protocol Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
Scan protocol Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
Description Prostate tumor-associated stromal cells.
Data processing Raw CEL intensity data were RMA normalized using R/Bioconductor
 
Submission date Sep 01, 2009
Last update date Sep 08, 2009
Contact name Ricardo Z.N. Vêncio
Organization name Universidade de São Paulo
Department Computing and Mathematics Department
Lab http://labpib.fmrp.usp.br
Street address Av. Bandeirantes, 3900
City Ribeirao Preto
State/province SP
ZIP/Postal code 14049-900
Country Brazil
 
Platform ID GPL570
Series (1)
GSE17906 Gene expression down-regulation in prostate tumor-associated stromal cells involves organ-specific genes
Relations
Reanalyzed by GSE20125

Data table header descriptions
ID_REF
VALUE RMA-normalized log2 signal

Data table
ID_REF VALUE
1007_s_at 9.16793467298644
1053_at 6.91340422878901
117_at 11.9782441390132
121_at 7.52853819641943
1255_g_at 3.50541198746391
1294_at 6.95362355882857
1316_at 5.74375732168299
1320_at 6.0567265082147
1405_i_at 5.42229954115382
1431_at 3.83273176060853
1438_at 6.81139419239756
1487_at 8.29217346677353
1494_f_at 5.44540578363103
1552256_a_at 6.7214262080967
1552257_a_at 6.21620588330945
1552258_at 5.59335025279275
1552261_at 4.95910879315657
1552263_at 5.60286779134644
1552264_a_at 6.36736708922803
1552266_at 4.77803790825057

Total number of rows: 54675

Table truncated, full table size 1478 Kbytes.




Supplementary file Size Download File type/resource
GSM447178.CEL.gz 5.5 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap