|
| Status |
Public on Apr 03, 2023 |
| Title |
hHEP_day 4_TBBPS-1 |
| Sample type |
SRA |
| |
|
| Source name |
hESCs hepatic differentiation
|
| Organism |
Homo sapiens |
| Characteristics |
differentiation stage: endoderm
original strain: hESCs-H9
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The samples were extracted with trizol reagent according to the manufactures guidance
Library was constructed by Beijin Genomic Institude (BGI) with the standard operating procedure
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
Base calling were conducted with BGISEQ-500
Data were filtered with SOAPnuke and trimmomatic software for removing the reads of adapter sequences; the reads containing more than 5% unknown nucleotides; the reads contain more than 20% low quality nucleotides, whose quality is lower than 10. The clean data generated by filtration were saved as fastq format and subjected to further analysis
The clean data were alignment to the reference sequence and the expected counts were calculated by using Bowtie2 (ver. 2.3.4.1-1) and RSEM (ver. 1.2.28)
Genome_build: GCA_000001405.28_hg38.p13
Supplementary_files_format_and_content: .xls file with table showing expected counts of genes in different samples
|
| |
|
| Submission date |
Apr 12, 2020 |
| Last update date |
Apr 03, 2023 |
| Contact name |
Renjun Yang |
| E-mail(s) |
rjyang@rcees.ac.cn
|
| Phone |
18811781909
|
| Organization name |
RCEES
|
| Street address |
双清路18号林业大学北门
|
| City |
北京市 |
| State/province |
北京 |
| ZIP/Postal code |
100085 |
| Country |
China |
| |
|
| Platform ID |
GPL23227 |
| Series (1) |
| GSE148518 |
TBBPA, TBBPS and TCBPA disrupt hESCs hepatic differentiation and promote the proliferation of differentiated cells seemingly via up-regulating the FGF10 signaling pathway |
|
| Relations |
| BioSample |
SAMN14586958 |
| SRA |
SRX8109931 |
