|
| Status |
Public on Dec 31, 2020 |
| Title |
cSLE skin lesion [020_20A_cSLE] |
| Sample type |
RNA |
| |
|
| Source name |
Skin biopsy from human
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Skin biopsy
disease: cSLE skin lesion
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the E.N.Z.A. FFPE RNA Kit (Omega Bio-tek) (Norcross, GA) following manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the NuGEN Encore Biotin Module (Encore Biotin Module Manual, P/N M01111 v6) following the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Biotin labeled cDNA was fragmented and hybridized to Affymetrix Human Gene 2.1 ST arrays (Santa Clara, CA) according to the manufacturer protocol.
|
| Scan protocol |
GeneChip arrays were scanned by using the Affymetrix GeneTitan system (software version 3.2.4.1515) according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression data from human childhood-onset lupus lesional skin biopsy
|
| Data processing |
The data were analyzed using R software version 3.6.1 using custom CDF version 23.0.0 and modified Affymetrix_1.58.0 package from BrainArray: RMA method and Human Entrez Gene custom CDF annotation version 23 were used for the normalization.
|
| |
|
| Submission date |
Apr 17, 2020 |
| Last update date |
Jan 01, 2021 |
| Contact name |
Celine C Berthier |
| Organization name |
University of Michigan
|
| Street address |
1150 W. Medical Center Dr, MSRB2
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL28426 |
| Series (1) |
| GSE148810 |
Gene expression of skin biopsie samples from juvenile myositis (JM) and childhood-onset lupus (cSLE). |
|
