|
| Status |
Public on Sep 05, 2009 |
| Title |
S010 |
| Sample type |
RNA |
| |
|
| Source name |
prostate, S010
|
| Organism |
Homo sapiens |
| Characteristics |
patient id: P093
sample type (t/tumor; biopsy; ctrl/autopsy sample): T
percentage of tumor: 47.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QiaGen Rneasy extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
50 microgram (10 microgram for biopsy tissue) of total RNA samples of each cases were amplified and labeled with biotin using Ovation RNA Amplification System V2 and FL-Ovation cDNA Biotin Module V2 (NuGen Inc.).
|
| |
|
| Hybridization protocol |
Following Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner.
|
| Description |
prostate
|
| Data processing |
The data were analyzed and normalized with RMA.
|
| |
|
| Submission date |
Sep 03, 2009 |
| Last update date |
Sep 04, 2009 |
| Contact name |
Yipeng Wang |
| E-mail(s) |
ywang@sdibr.org
|
| Phone |
8585813960
|
| Organization name |
Vaccine Research Institute of San Diego
|
| Street address |
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE17951 |
Gene expression analysis of prostate cancer samples using Affymetrix U133Plus2 array |
|
