|
| Status |
Public on Oct 13, 2021 |
| Title |
CACO_JAMA_3 |
| Sample type |
RNA |
| |
|
| Source name |
CACO_JAMA_3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CACO
genotype: JAM-A silencing
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from FFPE tissue using Recover ALL kit (Ambion)
|
| Label |
NA
|
| Label protocol |
RNA was not labeled; instead, RNA (33 ng/ul) was pre-processed following the NanoString manufacturer's protocol.
|
| |
|
| Hybridization protocol |
RNA was hybridized to capture probes and detection probes using the NanoString nCounter system according to the manufacturer's protocol. Detection probes were provided by the manufacturer and were labeled with a proprietary fluorescent barcode system.
|
| Scan protocol |
Scanning was conducted using the NanoString confocal microscope scanner/Digital Analyzer according to manufacturer's instructions.
|
| Data processing |
Data were collected as NanoString RCC files and provided as an Excel spreadsheet.
|
| |
|
| Submission date |
Apr 30, 2020 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Luciano Cascione |
| E-mail(s) |
luciano.cascione@ior.usi.ch
|
| Organization name |
IOR
|
| Department |
Lymphoma & Genomics Research Program
|
| Street address |
Via Chiesa 5
|
| City |
Bellinzona |
| State/province |
Ticino |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL19956 |
| Series (1) |
| GSE149658 |
MicroRNA-21-induced loss of Junctional Adhesion Molecule-A promotes activation of oncogenic pathways, progression and metastasis in colorectal cancer |
|
