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Status |
Public on Sep 10, 2009 |
Title |
14 h post-estradiol,biological rep3 |
Sample type |
RNA |
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Source name |
G1E ER4 cell, after estradiol induction, 14hr
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Organism |
Mus musculus |
Characteristics |
cell type: G1E ER4
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Treatment protocol |
G1E-ER4 cells growing in log phase were induced to differentiate for 0, 3, 7, 14, 21, or 30 hours with 20nM estradiol.
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Growth protocol |
G1E-ER4 cells were cultured as described previously. Weiss MJ, Yu C, Orkin SH. Erythroid-cell-specific properties of transcription factor GATA-1 revealed by phenotypic rescue of a gene-targeted cell line. Mol Cell Biol. 1997;17: 1642-1651.
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Extracted molecule |
total RNA |
Extraction protocol |
RNA from G1E-ER4 cells was extracted using Trizol reagent (Invitrogen, Carlsbad, CA).
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Label |
biotin
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Label protocol |
All protocols were conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
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Hybridization protocol |
All protocols were conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
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Scan protocol |
All protocols were conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual
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Description |
none
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Data processing |
The data were analyzed with Bioconductor package in R, justRMA() functionwas used for the normalization.
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Submission date |
Sep 09, 2009 |
Last update date |
Sep 09, 2009 |
Contact name |
Ross Hardison |
E-mail(s) |
rch8@psu.edu
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Organization name |
Pennsylvania State University
|
Street address |
303 Wartik Lab
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City |
University Park |
State/province |
PA |
ZIP/Postal code |
16802 |
Country |
USA |
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Platform ID |
GPL1261 |
Series (1) |
GSE18042 |
Erythroid differentiation: G1E model |
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