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Sample GSM451146 Query DataSets for GSM451146
Status Public on Sep 10, 2009
Title 14 h post-estradiol,biological rep3
Sample type RNA
 
Source name G1E ER4 cell, after estradiol induction, 14hr
Organism Mus musculus
Characteristics cell type: G1E ER4
Treatment protocol G1E-ER4 cells growing in log phase were induced to differentiate for 0, 3, 7, 14, 21, or 30 hours with 20nM estradiol.
Growth protocol G1E-ER4 cells were cultured as described previously. Weiss MJ, Yu C, Orkin SH. Erythroid-cell-specific properties of transcription factor GATA-1 revealed by phenotypic rescue of a gene-targeted cell line. Mol Cell Biol. 1997;17: 1642-1651.
Extracted molecule total RNA
Extraction protocol RNA from G1E-ER4 cells was extracted using Trizol reagent (Invitrogen, Carlsbad, CA).
Label biotin
Label protocol All protocols were conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
 
Hybridization protocol All protocols were conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
Scan protocol All protocols were conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual
Description none
Data processing The data were analyzed with Bioconductor package in R, justRMA() functionwas used for the normalization.
 
Submission date Sep 09, 2009
Last update date Sep 09, 2009
Contact name Ross Hardison
E-mail(s) rch8@psu.edu
Organization name Pennsylvania State University
Street address 303 Wartik Lab
City University Park
State/province PA
ZIP/Postal code 16802
Country USA
 
Platform ID GPL1261
Series (1)
GSE18042 Erythroid differentiation: G1E model

Data table header descriptions
ID_REF
VALUE Log2 tranformed normalized signals.

Data table
ID_REF VALUE
1415670_at 8.932512504
1415671_at 9.928745922
1415672_at 9.688864729
1415673_at 8.704208432
1415674_a_at 9.615471162
1415675_at 10.64389853
1415676_a_at 11.49481896
1415677_at 9.034374916
1415678_at 11.63177459
1415679_at 11.7246778
1415680_at 9.462783799
1415681_at 10.29780548
1415682_at 8.44702956
1415683_at 11.41632029
1415684_at 7.736938085
1415685_at 9.21905569
1415686_at 10.29893357
1415687_a_at 8.750535232
1415688_at 9.72045409
1415689_s_at 8.437743893

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM451146.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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