|
| Status |
Public on Sep 10, 2009 |
| Title |
21 h post-estradiol,biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
G1E ER4 cell, after estradiol induction, 21hr
|
| Organism |
Mus musculus |
| Characteristics |
cell type: G1E ER4
|
| Treatment protocol |
G1E-ER4 cells growing in log phase were induced to differentiate for 0, 3, 7, 14, 21, or 30 hours with 20nM estradiol.
|
| Growth protocol |
G1E-ER4 cells were cultured as described previously. Weiss MJ, Yu C, Orkin SH. Erythroid-cell-specific properties of transcription factor GATA-1 revealed by phenotypic rescue of a gene-targeted cell line. Mol Cell Biol. 1997;17: 1642-1651.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from G1E-ER4 cells was extracted using Trizol reagent (Invitrogen, Carlsbad, CA).
|
| Label |
biotin
|
| Label protocol |
All protocols were conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
|
| |
|
| Hybridization protocol |
All protocols were conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
|
| Scan protocol |
All protocols were conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual
|
| Description |
none
|
| Data processing |
The data were analyzed with Bioconductor package in R, justRMA() functionwas used for the normalization.
|
| |
|
| Submission date |
Sep 09, 2009 |
| Last update date |
Sep 09, 2009 |
| Contact name |
Ross Hardison |
| E-mail(s) |
rch8@psu.edu
|
| Organization name |
Pennsylvania State University
|
| Street address |
303 Wartik Lab
|
| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE18042 |
Erythroid differentiation: G1E model |
|
