OASIS-/- mice were generated using a targeting vector containing a neomycin resistant gene. The OASIS targeting vector was electroporated into embryonic stem (ES) cells derived from 129/Sv (D3).
Growth protocol
These experiments were performed with the consent of the Animal Care and Use Committees of Miyazaki University and Osaka University.
Extracted molecule
total RNA
Extraction protocol
Experimental sample RNAs from calvaria at postnatal 4 days were isolated using RNeasy (QIAGEN).
Label
Cy3
Label protocol
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See http://www.nimblegen.com/.
Hybridization protocol
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See http://www.nimblegen.com/.
Scan protocol
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See http://www.nimblegen.com/.
Description
Gene expression data from OASIS KO calvaria
Data processing
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 2003, 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 2003, 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
