|
| Status |
Public on Sep 10, 2009 |
| Title |
KO |
| Sample type |
RNA |
| |
|
| Source name |
OASIS KO calvaria
|
| Organism |
Mus musculus |
| Characteristics |
tissue: KO calvaria
|
| Treatment protocol |
OASIS-/- mice were generated using a targeting vector containing a neomycin resistant gene. The OASIS targeting vector was electroporated into embryonic stem (ES) cells derived from 129/Sv (D3).
|
| Growth protocol |
These experiments were performed with the consent of the Animal Care and Use Committees of Miyazaki University and Osaka University.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Experimental sample RNAs from calvaria at postnatal 4 days were isolated using RNeasy (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
Labeling was performed byMIYAZAKI Prefectural Industrial Support Foundation.
|
| |
|
| Hybridization protocol |
Affymetrix standard procedure
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000.
|
| Description |
Gene expression data from OASIS KO calvaria
|
| Data processing |
The data were analyzed with Expression Console version 1.1 using Affymetrix RMA algorithm and quantile as normalization method.
|
| |
|
| Submission date |
Sep 10, 2009 |
| Last update date |
Sep 10, 2009 |
| Contact name |
Kazunori Imaizumi |
| E-mail(s) |
imaizumi@med.miyazaki-u.ac.jp
|
| Organization name |
Division of Molecular and Cellular Biology, Department of Anatomy, Faculty of Medicine, University of Miyazaki
|
| Street address |
5200 Kihara, Kiyotake
|
| City |
Miyazaki |
| ZIP/Postal code |
889-1692 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE18062 |
Microarray of RNA from calvaria in WT and OASIS-/- (KO) mice |
|
