|
| Status |
Public on Jan 01, 2010 |
| Title |
WT Fed L4, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Wild Type L4 animal
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: wild type
stage: L4 larvae
|
| Treatment protocol |
Fed L4 animals were wild type or one of two mutant strains: loss of the O-GlcNAc transferase ogt-1(ok430) and loss of the O-GlcNAcase oga-1(ok1207)
|
| Growth protocol |
Fed L4 larval stage animals were isolated from wild type and mutant strains
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from 20 L4 animals was performed and cDNA generated and amplified with T7 polymerase one round as described in Fukushige et al., 2006
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from the total amplified cDNA reaction
|
| |
|
| Hybridization protocol |
C. elegans whole genome expression array chips were washed and stained in the Affymetrix Fluidics Station 400s
|
| Scan protocol |
Chips were scanned with an Affymetrix Scanner with standard settings
|
| Description |
N2C_Fed_L4
|
| Data processing |
Data was analyzed by Micrarray Suite 5.0 (MAS5) and by RMA using the program Partek
|
| |
|
| Submission date |
Sep 16, 2009 |
| Last update date |
Sep 16, 2009 |
| Contact name |
Michael Krause |
| E-mail(s) |
mwkrause@helix.nih.gov
|
| Phone |
(301) 402-4633
|
| Organization name |
NIH
|
| Department |
LMB/NIDDK
|
| Lab |
Krause
|
| Street address |
5 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (2) |
| GSE18131 |
Expression from C. elegans L4 animals |
| GSE18132 |
Dynamic O-GlcNAc cycling at promoters of C. elegans genes regulating Longevity, Stress, and Immunity |
|
