|
Status |
Public on Dec 31, 2009 |
Title |
age-1(mg44)_rep1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
young adult hermaphrodite
|
Organism |
Caenorhabditis elegans |
Characteristics |
strain: age-1(mg44) mutant
|
Growth protocol |
Eggs obtained by bleach prep of gravid hermaphrodites and hatched overnight in S basal to synchronize at L1. L1's were plated onto NG agar with OP50 food and grown 72 hours to L4/young adult stage at 20˚C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with Absolutely RNA miniprep kit (Stratagene, La Jolla, CA)
|
Label |
Cy3
|
Label protocol |
cDNA was labeled with the Quick Amp 2 Color Labeling Kit (Agilent Technologies, Santa Clara, CA)
|
|
|
Channel 2 |
Source name |
young adult hermaphrodite
|
Organism |
Caenorhabditis elegans |
Characteristics |
strain: wildtype (N2)
|
Growth protocol |
Eggs obtained by bleach prep of gravid hermaphrodites and hatched overnight in S basal to synchronize at L1. L1's were plated onto NG agar with OP50 food and grown 72 hours to L4/young adult stage at 20˚C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with Absolutely RNA miniprep kit (Stratagene, La Jolla, CA)
|
Label |
Cy5
|
Label protocol |
cDNA was labeled with the Quick Amp 2 Color Labeling Kit (Agilent Technologies, Santa Clara, CA)
|
|
|
|
Hybridization protocol |
standard Agilent protocol
|
Scan protocol |
standard Agilent protocol
|
Description |
n/a
|
Data processing |
Probes with raw fluorescence signals above 100 in wildtype were selected for further analysis as being reliable signals. Expression ratios for each gene were calculated in all 3 strains with respect to wildtype controls. Expression ratios were calculated as z-scores for a statistical evaluation of relative expression and as fold-changes for standardized displays of relative expression. To determine fold-change, raw fluorescence values were first normalized by dividing the fluorescence intensity for a given sample by the average intensity of all samples for the array. Fold-change ratios were then determined by dividing mean normalized value for all replicates of test versus control conditions.
|
|
|
Submission date |
Sep 21, 2009 |
Last update date |
Sep 21, 2009 |
Contact name |
Catherine Wolkow |
E-mail(s) |
wolkowca@mail.nih.gov
|
Organization name |
NIA IRP, NIH
|
Street address |
251 Bayview Blvd
|
City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21224 |
Country |
USA |
|
|
Platform ID |
GPL2875 |
Series (1) |
GSE18200 |
Gene expression C. elegans age-1 mutants with neuron- or intestine-restricted age-1 activity |
|