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Sample GSM455141 Query DataSets for GSM455141
Status Public on Dec 31, 2009
Title age-1(mg44)_rep1
Sample type RNA
 
Channel 1
Source name young adult hermaphrodite
Organism Caenorhabditis elegans
Characteristics strain: age-1(mg44) mutant
Growth protocol Eggs obtained by bleach prep of gravid hermaphrodites and hatched overnight in S basal to synchronize at L1. L1's were plated onto NG agar with OP50 food and grown 72 hours to L4/young adult stage at 20˚C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with Absolutely RNA miniprep kit (Stratagene, La Jolla, CA)
Label Cy3
Label protocol cDNA was labeled with the Quick Amp 2 Color Labeling Kit (Agilent Technologies, Santa Clara, CA)
 
Channel 2
Source name young adult hermaphrodite
Organism Caenorhabditis elegans
Characteristics strain: wildtype (N2)
Growth protocol Eggs obtained by bleach prep of gravid hermaphrodites and hatched overnight in S basal to synchronize at L1. L1's were plated onto NG agar with OP50 food and grown 72 hours to L4/young adult stage at 20˚C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with Absolutely RNA miniprep kit (Stratagene, La Jolla, CA)
Label Cy5
Label protocol cDNA was labeled with the Quick Amp 2 Color Labeling Kit (Agilent Technologies, Santa Clara, CA)
 
 
Hybridization protocol standard Agilent protocol
Scan protocol standard Agilent protocol
Description n/a
Data processing Probes with raw fluorescence signals above 100 in wildtype were selected for further analysis as being reliable signals. Expression ratios for each gene were calculated in all 3 strains with respect to wildtype controls. Expression ratios were calculated as z-scores for a statistical evaluation of relative expression and as fold-changes for standardized displays of relative expression. To determine fold-change, raw fluorescence values were first normalized by dividing the fluorescence intensity for a given sample by the average intensity of all samples for the array. Fold-change ratios were then determined by dividing mean normalized value for all replicates of test versus control conditions.
 
Submission date Sep 21, 2009
Last update date Sep 21, 2009
Contact name Catherine Wolkow
E-mail(s) wolkowca@mail.nih.gov
Organization name NIA IRP, NIH
Street address 251 Bayview Blvd
City Baltimore
State/province MD
ZIP/Postal code 21224
Country USA
 
Platform ID GPL2875
Series (1)
GSE18200 Gene expression C. elegans age-1 mutants with neuron- or intestine-restricted age-1 activity

Data table header descriptions
ID_REF
VALUE normalized log2 (mutant/wildtype)

Data table
ID_REF VALUE
3 -0.029089401
8 0.100904961
9 -0.459210583
10 0.206058108
11 -0.08902813
13 -0.016910442
15 0.229103942
16 -1.312533328
18 -0.363668594
20 0.020712338
22 -0.113058608
25 0.508238529
26 0.062492059
27 0.067536858
31 -0.07732687
32 0.012247923
33 0.17961141
34 -0.115714458
37 -0.026046458
38 0.090505783

Total number of rows: 16775

Table truncated, full table size 291 Kbytes.




Supplementary file Size Download File type/resource
GSM455141_age-1_mg44_rep1_251279510595_S01.txt.gz 5.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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