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Sample GSM455143 Query DataSets for GSM455143
Status Public on Dec 31, 2009
Title neuron_age-1_rep1
Sample type RNA
 
Channel 1
Source name young adult hermaphrodite
Organism Caenorhabditis elegans
Characteristics strain: neuron-restricted age-1 expression
Growth protocol Eggs obtained by bleach prep of gravid hermaphrodites and hatched overnight in S basal to synchronize at L1. L1's were plated onto NG agar with OP50 food and grown 72 hours to L4/young adult stage at 20˚C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with Absolutely RNA miniprep kit (Stratagene, La Jolla, CA)
Label Cy3
Label protocol cDNA was labeled with the Quick Amp 2 Color Labeling Kit (Agilent Technologies, Santa Clara, CA)
 
Channel 2
Source name young adult hermaphrodite
Organism Caenorhabditis elegans
Characteristics strain: wildtype (N2)
Growth protocol Eggs obtained by bleach prep of gravid hermaphrodites and hatched overnight in S basal to synchronize at L1. L1's were plated onto NG agar with OP50 food and grown 72 hours to L4/young adult stage at 20˚C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with Absolutely RNA miniprep kit (Stratagene, La Jolla, CA)
Label Cy5
Label protocol cDNA was labeled with the Quick Amp 2 Color Labeling Kit (Agilent Technologies, Santa Clara, CA)
 
 
Hybridization protocol standard Agilent protocol
Scan protocol standard Agilent protocol
Description n/a
Data processing Probes with raw fluorescence signals above 100 in wildtype were selected for further analysis as being reliable signals. Expression ratios for each gene were calculated in all 3 strains with respect to wildtype controls. Expression ratios were calculated as z-scores for a statistical evaluation of relative expression and as fold-changes for standardized displays of relative expression. To determine fold-change, raw fluorescence values were first normalized by dividing the fluorescence intensity for a given sample by the average intensity of all samples for the array. Fold-change ratios were then determined by dividing mean normalized value for all replicates of test versus control conditions.
 
Submission date Sep 21, 2009
Last update date Sep 21, 2009
Contact name Catherine Wolkow
E-mail(s) wolkowca@mail.nih.gov
Organization name NIA IRP, NIH
Street address 251 Bayview Blvd
City Baltimore
State/province MD
ZIP/Postal code 21224
Country USA
 
Platform ID GPL2875
Series (1)
GSE18200 Gene expression C. elegans age-1 mutants with neuron- or intestine-restricted age-1 activity

Data table header descriptions
ID_REF
VALUE normalized log2 (mutant/wildtype)

Data table
ID_REF VALUE
3 0.243997796
8 -0.008285062
9 -0.778789088
10 0.157850364
11 -0.137150779
13 0.102254091
15 0.337501753
16 -1.525569364
18 -0.107541863
20 0.136476569
22 -0.149071645
25 0.82958971
26 0.021241937
27 0.000140993
31 -0.04377339
32 -0.002370612
33 0.123599261
34 -0.578587532
37 -0.05534392
38 0.005914379

Total number of rows: 16775

Table truncated, full table size 292 Kbytes.




Supplementary file Size Download File type/resource
GSM455143_neuron_age-1_rep1_251279510597_S01.txt.gz 5.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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