|
| Status |
Public on Sep 09, 2020 |
| Title |
KYSE510_20 µM SFE-treated cells |
| Sample type |
RNA |
| |
|
| Source name |
KYSE510 with 20 µM SFE treatment
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: KYSE510
cell type: human esophageal cancer cell line
treatment: 20 µM Sulforaphene (SFE)
|
| Treatment protocol |
KYSE510 cells were seeded in 6-well plates with 20 µM SFE treatment or DMSO treatment as the negative control.After cultured for 48h, cells were treated with trizol reagent to extract total RNA.
|
| Growth protocol |
KYSE510 cells were cultured in RPMI-1640 medium with 10% fetal bovine serum, 100 units/mL penicillin and 100 mg/mL streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled and purified by Affymetrix FL-Ovation™ cDNA Biotin Module V2 according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.2 µg of biotin-labeled ss-cDNA were hybridized for 16 h at 45℃ on Affymetrix GeneChip Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from hunam esophageal cancer KYSE510 cells with 20 µM SFE treatment
|
| Data processing |
Command Console Software was used to control the scanner and summarize probe cell intensity data (CEL file generation) with default settings. Then raw data were normalized by Expression Console.
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| |
|
| Submission date |
May 19, 2020 |
| Last update date |
Sep 09, 2020 |
| Contact name |
sichong han |
| E-mail(s) |
hansichong@cicams.ac.cn
|
| Organization name |
beijing university of chemical technology
|
| Street address |
No. 15 North Third Ring East Road, Chaoyang District
|
| City |
beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE150891 |
Expression data in EC109 and KYSE510 cells |
|
