|
| Status |
Public on Oct 16, 2009 |
| Title |
Ess1 H164R Mutant34-rep5 |
| Sample type |
RNA |
| |
|
| Source name |
ess1(H164R) ts-mutants after a shift to 34°C for 2 hr
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype/variation: ess1(H164R) ts-mutant
|
| Growth protocol |
Cells were grown at in YPD at 30c and shifted to 34c for 2 hrs.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Epicenter RNA purification kit and Qiagen Rneasy columns
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix Guidelines
|
| |
|
| Hybridization protocol |
According to Affymetrix Guidelines
|
| Scan protocol |
According to Affymetrix Guidelines
|
| Description |
no additional information
|
| Data processing |
Tiling array software version 1.1 (TAS)
Analysed data provided as signal and p-value .bar files (see supplementary file on Series GSE17638 record).
Comparison of Ess1 H164R mutant vs WT is processed as bar files and can be visualized using IGB
|
| |
|
| Submission date |
Sep 24, 2009 |
| Last update date |
Sep 24, 2009 |
| Contact name |
Steven D Hanes |
| E-mail(s) |
hanes@wadsworth.org
|
| Phone |
518-473-4213
|
| Organization name |
Wadsworth Center
|
| Department |
Developmental Genetics
|
| Lab |
CMS5102
|
| Street address |
150 New Scotland Ave
|
| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12208 |
| Country |
USA |
| |
|
| Platform ID |
GPL7250 |
| Series (1) |
| GSE17638 |
The Ess1 prolyl isomerase is required for the transcription termination of small non-coding RNAs via Nrd1 pathway |
|
