|
Status |
Public on Nov 04, 2009 |
Title |
MEF RepSox 1 week rep2 |
Sample type |
RNA |
|
|
Source name |
Mouse embryonic fibroblasts (MEFs)
|
Organism |
Mus musculus |
Characteristics |
cell type: Embryonic Fibroblast Cells small molecule treatment: RepSox at 1 week
|
Treatment protocol |
Cells were treated with small molecules as described in Ichida et al. (Cell Stem Cell, 2009).
|
Growth protocol |
Cells were grown in a monolayer on gelatin-coated plates, either with or without an irradiated mouse embryonic fibroblast (MEF) feeder layer. They were given standard mES media containing either fetal bovine serum (FBS) or knock-out serum replacement (KSR).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent in accordance with the prescribed protocol.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina Total Prep RNA Amp kit.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Non-normalized data file: RAW_2.txt
|
Data processing |
The data were normalized using cubic-spline normalization with BeadStudio.
|
|
|
Submission date |
Sep 25, 2009 |
Last update date |
Sep 25, 2009 |
Contact name |
Esther Yesde Son |
E-mail(s) |
son@fas.harvard.edu
|
Phone |
617-496-5611
|
Fax |
617-384-5611
|
Organization name |
Harvard University
|
Department |
Stem Cells and Regenerative Biology
|
Lab |
Eggan
|
Street address |
7 Divinity Avenue
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02138 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE18286 |
Global gene expression analysis of (1) chemically reprogrammed iPS cells, and (2) chemical-treated cells |
|