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Sample GSM4585002 Query DataSets for GSM4585002
Status Public on Feb 18, 2021
Title YoungTreg_naive_5_RNAseq
Sample type SRA
 
Source name Lung Treg cells (CD3e+CD4+CD25hiFR4+)
Organism Mus musculus
Characteristics age: 2-months old
exposure: naïve
cell type: Lung Treg cells (CD3e+CD4+CD25hiFR4+)
Extracted molecule total RNA
Extraction protocol RNA was extracted from sorted cells using the Qiagen AllPrep DNA/RNA Micro Kit.
NEB Next RNA Ultra, with polyA enrichment (Total, ribodRNA) and dual indexes
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Basecalls performed using Illumina RTA2 software.
BCL2FASTQ v2.17.1.14 was used to convert bcl files to fastq files (default parameters).
Fastq files were aligned to the mm10 reference genome using Tophat v.2.1.0, and SeqMonk v1.45.4 was used to generate gene and transcript expression levels for protein-coding genes.
Genome_build: GRCm38/mm10
Supplementary_files_format_and_content: Tab-delimited text file including raw counts values for each sample generated for uniquely mapped reads over exons was obtained using SeqMonk v1.45.4.
 
Submission date Jun 01, 2020
Last update date Feb 18, 2021
Contact name Benjamin D. Singer
E-mail(s) benjamin-singer@northwestern.edu
Organization name Northwestern University Feinberg School of Medicine
Street address 303 E. Superior St., Simpson Querrey 5th floor
City Chicago
State/province Illinois
ZIP/Postal code 60611
Country USA
 
Platform ID GPL19057
Series (2)
GSE151542 Aging imparts cell-autonomous dysfunction to regulatory T cells during recovery from influenza pneumonia [RNA-seq]
GSE151543 Aging imparts cell-autonomous dysfunction to regulatory T cells during recovery from influenza pneumonia
Relations
BioSample SAMN15074569
SRA SRX8439654

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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