|
| Status |
Public on May 23, 2023 |
| Title |
Control-2 |
| Sample type |
RNA |
| |
|
| Source name |
B16F10 mouse melanoma cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Chd4+/+
|
| Treatment protocol |
Mi-2β-knockout and non-target control B16F10 cell lines were treated with IFN-γ (10ng/mL) for 24 hours in complete DMEM media.
|
| Growth protocol |
Mi-2β-knockout and non-target control B16F10 cell lines were cultured in complete DMEM media (10% FBS and 100U/ml of Penicillin-Streptomycin).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from Mi-2β knockout and control B16F10 cells with the RNeasy Mini Kit (74104) (Qiagen, Hilden, Germany) according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed using the WT Plus reagent kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Mouse Gene 2.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
| Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
|
| Description |
Gene expression data from Chd4+/+ B16F10 mouse melanoma cells.
|
| Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.58.0), using R version 3.5.1 and the Brainarray mogene20stmmentrezgcdf R package (version 17.0.0).
|
| |
|
| Submission date |
Jun 02, 2020 |
| Last update date |
May 23, 2023 |
| Contact name |
Boston University Microarray and Sequencing Resource |
| E-mail(s) |
msrdata@bu.edu
|
| Organization name |
Boston University
|
| Department |
Microarray and Sequencing Resource
|
| Street address |
72 East Concord Street, E631
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL17791 |
| Series (1) |
| GSE151640 |
Mi-2β knockout enhanced response of B16F10 cells to IFN-γ |
|
