|
| Status |
Public on Oct 08, 2009 |
| Title |
9_P154529 |
| Sample type |
RNA |
| |
|
| Source name |
CD14+ blood monocytes
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: CD14+ monocytes
fatigue status: high
nrti treatment status: Yes
hiv status: HIV
|
| Treatment protocol |
CD14+ monocytes were isolated by aphoresis followed by negative sorting by antibodys to other PBMC fractions.
|
| Growth protocol |
Not applicable.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Mini column kit from Qiagen.
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol.
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol.
|
| Scan protocol |
Affymetrix standard protocol.
|
| Description |
CIS #: P154529
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Oct 07, 2009 |
| Last update date |
Oct 07, 2009 |
| Contact name |
Lawrence D Adams |
| E-mail(s) |
ladams@u.washington.edu
|
| Phone |
206 616-0145
|
| Organization name |
University of Washington
|
| Department |
BNHS
|
| Lab |
Voss
|
| Street address |
1959 NE Pacific Street
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL9392 |
| Series (1) |
| GSE18468 |
Fatigue-related HIV disease gene-networks identified in CD14+ cells isolated from HIV-infected patients |
|
