|
| Status |
Public on Nov 12, 2009 |
| Title |
+Ca_replicate_2_RMA_normalized |
| Sample type |
RNA |
| |
|
| Source name |
RNA isolated from primary human keratinocytes in differentiation conditions
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary keratinocytes
gender: male
tissue: foreskin
status: differentiated
|
| Growth protocol |
Keratinocytes were grown in Gibco SFM medium till 100% confluent with addition of 1.2 mM Ca2+ for 3 Days to induce differentiation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kits were used to isolate total RNA.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed by the Stanford P.A.N. facility.
|
| |
|
| Hybridization protocol |
Hybrization was performed by the Stanford P.A.N. facility.
|
| Scan protocol |
Scanning was performed by the Stanford P.A.N. facility.
|
| Description |
Human primary keratinocytes isolated from multiple foreskin samples; passage is less than 4.
|
| Data processing |
Raw Cel files was loaded into GeneSpring GX software and RMA normalized.
|
| |
|
| Submission date |
Oct 14, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Paul A Khavari |
| Organization name |
Stanford University
|
| Department |
Dermatology
|
| Lab |
Khavari Lab
|
| Street address |
259 Campus Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5168 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE18590 |
DNMT1 Maintains Progenitor Function in Self-Renewing Somatic Tissue |
|
| Relations |
| Reanalyzed by |
GSE119087 |
