|
| Status |
Public on Feb 27, 2003 |
| Title |
4hr MK-DG vs 4hr MK C+S-DG |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
DG Hippocampus, 4hr MK-DG was labeled with Cyanine-3 (green).
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| |
|
| Channel 2 |
| Source name |
DG Hippocampus, 4hr MK C+S-DG was labeled with Cyanine-5 (red)
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| |
|
| |
| Description |
MK: MK-801. Young male C57BL/6J mice received an injection of MK-801 (300 micro-g per g body weight). RNA from control mice were extracted 4hr after MK experimental mice received their last shock treatment.
MK C+S: MK-801 & Context + Shock: Young male C57BL/6J mice received an injection of MK-801 (300 micro-g per g body weight) 1hr prior to contextual fear conditioning that consisted of: Placement into a novel spatial context for 2 min. After 2 min, a 1 sec (0.5 mA) shock was administered through a floor grid. The 2 min-1 sec shock paradigm was repeated for a total of 3 shocks. 1 min after the last shock, animals were removed to their homecage. RNA was extracted 4hr after the last shock treatment.
|
| |
|
| Submission date |
Feb 25, 2003 |
| Last update date |
May 27, 2005 |
| Contact name |
Sangdun Choi |
| E-mail(s) |
schoi@caltech.edu
|
| Phone |
626-395-8732
|
| Fax |
626-796-7066
|
| Organization name |
California Institute of Technology
|
| Department |
Biology
|
| Lab |
Simon Lab
|
| Street address |
1200 E. California Blvd
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
|
| Platform ID |
GPL254 |
| Series (2) |
| GSE318 |
MK-DG vs MK C+S-DG |
| GSE1782 |
A Bioinformatics Analysis of Memory Consolidation Reveals Involvement of the Transcription Factor c-Rel |
|
| Data table header descriptions |
| ID_REF |
Feature number which correpond to the order of genes on the array |
| G_PROCESSED |
Processed Signal, after dye normalization and background subtraction, used for computation of log ratio |
| G_MEAN |
Green Channel Mean Raw Intensity |
| G_MEDIAN |
Green Channel Median Raw Intensity |
| G_SD |
Green Channel Standard Deviation |
| G_BG_MEAN |
Green Channel Mean Background Level |
| G_BG_MEDIAN |
Green Channel Median Background Level |
| G_BG_SD |
Green Channel Background Standard Deviation |
| R_PROCESSED |
Processed Signal, after dye normalization and background subtraction, used for computation of log ratio |
| R_MEAN |
Red Channel Mean Raw Intensity |
| R_MEDIAN |
Red Channel Median Raw Intensity |
| R_SD |
Red Channel Standard Deviation |
| R_BG_MEAN |
Red Channel Mean Background Level |
| R_BG_MEDIAN |
Red Channel Median Background Level |
| R_BG_SD |
Red Channel Background Standard Deviation |
| RATIO |
Log Ratio (base 10) |
| VALUE |
Processed Ratio (base 2) after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots. |
