|
| Status |
Public on Aug 21, 2020 |
| Title |
Arabidopsis_WT_L1_G5 (WGBS) |
| Sample type |
SRA |
| |
|
| Source name |
Arabidopsis_WT_L1_G5 (WGBS)
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
lineage: lineage 1 generation 5
tissue: leaf
ecotype: Col-0
|
| Growth protocol |
Seeds were planted and grown in 16-hour day lengths and samples were harvested from young above ground tissue. Tissue was flash frozen in liquid nitrogen and DNA was isolated using a Qiagen Plant DNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
MethylC-seq: prepared as previously described in (Urich et al. 2015).
|
| |
|
| Library strategy |
Bisulfite-Seq |
| Library source |
genomic |
| Library selection |
RANDOM |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
MethylC-seq: analysis was performed by Methylpy 1.3
Genome_build: Arabidopsis thaliana TAIR10 reference genome
Supplementary_files_format_and_content: methylome tab delimited text file, see "allc_column_header_descriptions.txt" for column headers
|
| |
|
| Submission date |
Jun 23, 2020 |
| Last update date |
Aug 22, 2020 |
| Contact name |
Robert J Schmitz |
| E-mail(s) |
schmitz@uga.edu
|
| Organization name |
University of Georgia
|
| Department |
Genetics
|
| Street address |
B416 Davison Life Sciences
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
| |
|
| Platform ID |
GPL19580 |
| Series (1) |
| GSE153055 |
AlphaBeta: Computational inference of epimutation rates and spectra from high-throughput DNA methylation data in plants |
|
| Relations |
| BioSample |
SAMN15351548 |
| SRA |
SRX8602648 |
