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Status
Public on Oct 22, 2009
Title
bonemarrow_stemcell_rep1
Sample type
RNA
Source name
Bone marrow
Organism
Homo sapiens Characteristics
growth protocol: Cultured in Low Glucose DMEM, 10% FBS, 100 U/ml penicillin, 0.1 mg/ml streptomycin
Growth protocol
Cells from 3 different donors per stem cell type were cultured under proliferative, non-differentiating conditions.
Extracted molecule
total RNA
Extraction protocol
Rneasy column purification
Label
biotin
Label protocol
GeneChip WT Terminal Labeling Kit
Hybridization protocol
17 hours at 45 degrees in Affymetrix hybridization oven at 60 rpm,washing and staining in Fluidics station 450 using stain cocktails: SAPE and antibody solution, (protocol FS450_0001)
Scan protocol
GeneChip scanner 3000 7G
Description
BM-MSC
Data processing
RMA + GC correction
Submission date
Oct 22, 2009
Last update date
Oct 22, 2009
Contact name
Bastiaan Jansen
E-mail(s)
bjhjansen@gmail.com Organization name
Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Center Nijmegen
Department
Tumor Immunology
Street address
PO Box 9101
City
Nijmegen
ZIP/Postal code
6500 HB
Country
Netherlands
Platform ID
GPL5175 Series (1)
GSE18698 Functional differences among human postnatal stem cells of different origin are reflected by their transcriptome
Data table header descriptions ID_REF VALUE RMA log2 intensities
Data table ID_REF VALUE 3881686
7.20698
2672712
6.07217
2842570
7.12125
3526544
3.3284
2902531
2.73076
2402942
4.81715
3382216
5.86232
3771800
7.47823
2427469
5.62522
2392945
3.98604
2453006
2.6163
3552083
3.38545
3416651
2.31594
2562821
7.54946
3721851
5.39058
3442176
5.91105
2477438
5.18491
2926969
5.12498
3026969
4.47868
3796335
5.25956
21980 341 Kbytes .Supplementary file Size Download File type/resource GSM464392_EX0601_02.CEL.gz
20.4 Mb
(ftp) (http) CEL
Processed data included within Sample table
