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Status
Public on Oct 22, 2009
Title
bonemarrow_stemcell_rep2
Sample type
RNA
Source name
Bone marrow
Organism
Homo sapiens Characteristics
growth protocol: Cultured in Low Glucose DMEM, 10% FBS, 100 U/ml penicillin, 0.1 mg/ml streptomycin
Growth protocol
Cells from 3 different donors per stem cell type were cultured under proliferative, non-differentiating conditions.
Extracted molecule
total RNA
Extraction protocol
Rneasy column purification
Label
biotin
Label protocol
GeneChip WT Terminal Labeling Kit
Hybridization protocol
17 hours at 45 degrees in Affymetrix hybridization oven at 60 rpm,washing and staining in Fluidics station 450 using stain cocktails: SAPE and antibody solution, (protocol FS450_0001)
Scan protocol
GeneChip scanner 3000 7G
Description
BM-MSC
Data processing
RMA + GC correction
Submission date
Oct 22, 2009
Last update date
Oct 22, 2009
Contact name
Bastiaan Jansen
E-mail(s)
bjhjansen@gmail.com Organization name
Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Center Nijmegen
Department
Tumor Immunology
Street address
PO Box 9101
City
Nijmegen
ZIP/Postal code
6500 HB
Country
Netherlands
Platform ID
GPL5175 Series (1)
GSE18698 Functional differences among human postnatal stem cells of different origin are reflected by their transcriptome
Data table header descriptions ID_REF VALUE RMA log2 intensities
Data table ID_REF VALUE 3881686
7.14263
2672712
6.15534
2842570
6.9109
3526544
3.53459
2902531
2.41432
2402942
4.37749
3382216
4.92955
3771800
7.30933
2427469
4.25659
2392945
4.63022
2453006
2.75664
3552083
3.58671
3416651
2.45651
2562821
7.62302
3721851
4.92101
3442176
5.79366
2477438
3.8155
2926969
5.41948
3026969
4.13468
3796335
5.16582
21980 341 Kbytes .Supplementary file Size Download File type/resource GSM464394_EX0705_01.CEL.gz
17.3 Mb
(ftp) (http) CEL
Processed data included within Sample table
