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Status
Public on Oct 22, 2009
Title
adipose_stemcell_rep3
Sample type
RNA
Source name
Adipose tissue
Organism
Homo sapiens Characteristics
growth protocol: Cultured in Low Glucose DMEM, 10% FBS, 100 U/ml penicillin, 0.1 mg/ml streptomycin
Growth protocol
Cells from 3 different donors per stem cell type were cultured under proliferative, non-differentiating conditions.
Extracted molecule
total RNA
Extraction protocol
Rneasy column purification
Label
biotin
Label protocol
GeneChip WT Terminal Labeling Kit
Hybridization protocol
17 hours at 45 degrees in Affymetrix hybridization oven at 60 rpm,washing and staining in Fluidics station 450 using stain cocktails: SAPE and antibody solution, (protocol FS450_0001)
Scan protocol
GeneChip scanner 3000 7G
Description
AdAS
Data processing
RMA + GC correction
Submission date
Oct 22, 2009
Last update date
Oct 22, 2009
Contact name
Bastiaan Jansen
E-mail(s)
bjhjansen@gmail.com Organization name
Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Center Nijmegen
Department
Tumor Immunology
Street address
PO Box 9101
City
Nijmegen
ZIP/Postal code
6500 HB
Country
Netherlands
Platform ID
GPL5175 Series (1)
GSE18698 Functional differences among human postnatal stem cells of different origin are reflected by their transcriptome
Data table header descriptions ID_REF VALUE RMA log2 intensities
Data table ID_REF VALUE 3881686
6.7249
2672712
6.06088
2842570
6.83537
3526544
2.82019
2902531
2.53887
2402942
4.34342
3382216
5.96286
3771800
6.30171
2427469
6.19463
2392945
4.74757
2453006
2.92621
3552083
3.67334
3416651
2.52938
2562821
7.67629
3721851
5.02408
3442176
5.58361
2477438
3.78945
2926969
3.97471
3026969
4.60224
3796335
5.47419
21980 341 Kbytes .Supplementary file Size Download File type/resource GSM464397_EX0705_04.CEL.gz
17.2 Mb
(ftp) (http) CEL
Processed data included within Sample table
