growth protocol: Cultured in Low Glucose DMEM, 30% FBS, 100 U/ml penicillin, 0.1 mg/ml streptomycin, 2 mM ultraglutamine, 10-7 M dexamethasone
Growth protocol
Cells from 3 different donors per stem cell type were cultured under proliferative, non-differentiating conditions.
Extracted molecule
total RNA
Extraction protocol
Rneasy column purification
Label
biotin
Label protocol
GeneChip WT Terminal Labeling Kit
Hybridization protocol
17 hours at 45 degrees in Affymetrix hybridization oven at 60 rpm,washing and staining in Fluidics station 450 using stain cocktails: SAPE and antibody solution, (protocol FS450_0001)
