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Sample GSM4645100 Query DataSets for GSM4645100
Status Public on Sep 30, 2020
Title RNA-seq cas9EZH2 3
Sample type SRA
 
Source name NKC_CRISPR/Cas9 EZH2
Organism Mus musculus
Characteristics cell line: NKC
cell type: PDAC (pancreatic ductal adenocarcinoma) cell line carrying pancreas specific-Kras mutation (KrasG12D)
transfected with: CRISPR/Cas9 EZH2
Growth protocol NKC cells were cultured in DMEM supplemented with 10% FCS and 1% NEAA.
Extracted molecule total RNA
Extraction protocol RNA was harvested using Trizol reagent
mRNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Raw reads were quality-checked using FastQC
Alignment against mm9 mouse reference genome was done by using TopHat2 (version 2.1.0)
Fragment per kilobase (FPKM) values were obtained by cuffnorm (version 2.2.1)
Differential gene expression was dervived by cuffdiff (version 2.2.1)
Genome_build: mm9
Supplementary_files_format_and_content: Differential gene expression
 
Submission date Jun 29, 2020
Last update date Sep 30, 2020
Contact name SHILPA PATIL
E-mail(s) shilpapatil528@gmail.com
Organization name UNIVERSITY MEDICAL CENTER GOETTINGEN
Street address ROBERT KOCH STRASSE 40
City GOETTINGEN
State/province No further selection needed
ZIP/Postal code 37075
Country Germany
 
Platform ID GPL13112
Series (2)
GSE153491 EZH2-GATA6 axis in Pancreatic ductal adenocarcinoma [RNA-Seq]
GSE153538 EZH2-GATA6 axis in Pancreatic ductal adenocarcinoma
Relations
BioSample SAMN15398727
SRA SRX8632452

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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