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Sample GSM4645131 Query DataSets for GSM4645131
Status Public on Aug 20, 2020
Title P14 Ifnar +/-, Flt3 +/+ HPC-2 [s4788_MGI0091_W2]
Sample type SRA
 
Source name P14 Ifnar +/-, Flt3 +/+ HPC
Organism Mus musculus
Characteristics age: P14
genotype: Ifnar +/-, Flt3 +/+
cell type: Hematopoietic progenitor cell
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy micro plus
Clontech SMRTr
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description processed data file: gene_level_read_counts.2nd_submission.txt.gz
Data processing The resulting sequencing reads were demultiplexed by index with a custom Python demultiplexing script and further demultiplexed by barcode with a custom Perl demultiplexing script.
The RNA-Seq reads were then aligned to the Mus musculus Ensembl release 76 top-level assembly with STAR version 3.0.4.b.
Gene counts were derived from the number of uniquely aligned unambiguous reads by Subread:featureCount version 1.4.5.
Sailfish version 0.6.3. was used to produce isoform estimated counts.
Genome_build: Ensemble R76
Supplementary_files_format_and_content: tab-delimited text files include read count values for each Sample
 
Submission date Jun 29, 2020
Last update date Aug 20, 2020
Contact name Jeffery Magee
E-mail(s) magee_j@kids.wustl.edu
Organization name Washington University School of Medicine
Department Internal Medicine - Cardiovascular Division
Street address 660 S Euclid Ave.
City St Louis
State/province Missouri
ZIP/Postal code 63110
Country USA
 
Platform ID GPL24247
Series (2)
GSE128759 Neonatal hematopoietic stem and progenitor cell ontogeny at single cell resolution (RNA-seq)
GSE128762 Neonatal hematopoietic stem and progenitor cell ontogeny at single cell resolution
Relations
BioSample SAMN15399413
SRA SRX8632963

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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