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Sample GSM4645148 Query DataSets for GSM4645148
Status Public on Aug 20, 2020
Title Adult HPC H3K27me3-2 [2893_1_B]
Sample type SRA
 
Source name Adult HPC H3K27me3
Organism Mus musculus
Characteristics age: 8 week old
cell type: Hematopoietic Progenitor Cell
histone_mark: H3K27ac
Treatment protocol N/A
Growth protocol N/A
Extracted molecule genomic DNA
Extraction protocol HSCs and HPCs were soted and then fixed for chromatin isolation per Schmidl et l., Nature Methods 10:963.
Chipmentation - Chromatin isolation with the indicated antibody followed by tagmentation with Tn5 as per Schmidl et l., Nature Methods 10:963.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2500
 
Description processed data in series file:
adult_HPC_k27_2893.fc.signal.bigwig
adult_HPC_k27_2893.filt.narrowPeak.gz
Data processing Sequencing reads were demultiplexed and aligned to mouse genome (mm10) by novoalign (versoin 3.04.06)
Signal strength relative to the control samples were generated using MACS2.
Optimal set of peaks were generated using the Kundaje lab AQUAS TF and histone ChIP-seq pipeline (https://github.com/kundajelab/chipseq_pipeline version 0.33)
Genome_build: GRCm38/mm10
Supplementary_files_format_and_content: bigwig, narrowPeak
 
Submission date Jun 29, 2020
Last update date Aug 20, 2020
Contact name Jeffery Magee
E-mail(s) magee_j@kids.wustl.edu
Organization name Washington University School of Medicine
Department Internal Medicine - Cardiovascular Division
Street address 660 S Euclid Ave.
City St Louis
State/province Missouri
ZIP/Postal code 63110
Country USA
 
Platform ID GPL17021
Series (2)
GSE128760 Neonatal hematopoietic stem and progenitor cell ontogeny at single cell resolution (ChIP-seq)
GSE128762 Neonatal hematopoietic stem and progenitor cell ontogeny at single cell resolution
Relations
BioSample SAMN15399451
SRA SRX8632980

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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