|
Status |
Public on Sep 30, 2020 |
Title |
H3K27me3 ChIP shctrl 2 |
Sample type |
SRA |
|
|
Source name |
Pancreatic ductal adenocarcinoma
|
Organism |
Mus musculus |
Characteristics |
cell line: NKC treatment: shRNA control
|
Growth protocol |
NKC cells were cultured in DMEM supplemented with 10% FCS and 1% NEAA.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
H3K27me3 ChIP shctrl 2
|
Data processing |
Raw reads were quality-checked using FastQC Alignment against mm9 mouse reference genome was done by using BOWTIE2 version 0.4 Peak calling was performed by MACS2 version 2.1.0 Diffbind (Bioconductor R package) was utilized for differential binding analysis Genome_build: mm9 Supplementary_files_format_and_content: bigwig
|
|
|
Submission date |
Jun 30, 2020 |
Last update date |
Sep 30, 2020 |
Contact name |
SHILPA PATIL |
E-mail(s) |
shilpapatil528@gmail.com
|
Organization name |
UNIVERSITY MEDICAL CENTER GOETTINGEN
|
Street address |
ROBERT KOCH STRASSE 40
|
City |
GOETTINGEN |
State/province |
No further selection needed |
ZIP/Postal code |
37075 |
Country |
Germany |
|
|
Platform ID |
GPL13112 |
Series (2) |
GSE153537 |
EZH2-GATA6 axis in Pancreatic ductal adenocarcinoma [ChIP-Seq] |
GSE153538 |
EZH2-GATA6 axis in Pancreatic ductal adenocarcinoma |
|
Relations |
BioSample |
SAMN15403438 |
SRA |
SRX8639422 |