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Sample GSM4672247 Query DataSets for GSM4672247
Status Public on Nov 12, 2020
Title WT_ChIPseq_H3K4me3
Sample type SRA
 
Source name Mycelium
Organism Neurospora crassa
Characteristics genotype: WT
tissue: Mycelial cultures
chip antibody: H3K4me3 (Abcam Cat# ab1012)
Treatment protocol ATAC-seq, ChIP-seq
Growth protocol vogel's 1.5% sucrose
Extracted molecule genomic DNA
Extraction protocol Illumina TruSeq
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
 
Data processing Trimmed with TrimGalore! Version version 4.0 parameters --length 20 --fastqc
alignment with bwa mem version 0.7.15/ parameters -M or bowtie2 version 2.4.1/parameters --verysensitive
conversion to bam and sorted and indexed with samtools version 1.3.1/
bigwigs made with DeepTools 3.3.1/ parameters --normalizeUsing CPM
Deeptools for creation of heatmaps and metaplots
Genome_build: GCA_000182925.2 (NC12)
Supplementary_files_format_and_content: bigwig
 
Submission date Jul 15, 2020
Last update date Nov 14, 2020
Contact name Zachary A Lewis
E-mail(s) zacharyaustinlewis@gmail.com
Organization name University of Georgia
Department Microbiology
Lab Lewis
Street address 1000 Cedar St. Biosciences Bldg
City Athens
State/province GA
ZIP/Postal code 30602
Country USA
 
Platform ID GPL20660
Series (1)
GSE154497 Chromatin accessibility profiling in Neurospora crassa reveals features associated with accessible and inaccessible chromatin
Relations
BioSample SAMN15544542
SRA SRX8737831

Supplementary file Size Download File type/resource
GSM4672247_SortedSized106-47_ChIP_S2_WT_K4me3_Rep1_Nc_ON.CPM.bw 1.3 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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