|
Status |
Public on Nov 12, 2020 |
Title |
WT_ChIPseq_H3K4me3 |
Sample type |
SRA |
|
|
Source name |
Mycelium
|
Organism |
Neurospora crassa |
Characteristics |
genotype: WT tissue: Mycelial cultures chip antibody: H3K4me3 (Abcam Cat# ab1012)
|
Treatment protocol |
ATAC-seq, ChIP-seq
|
Growth protocol |
vogel's 1.5% sucrose
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Illumina TruSeq
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Trimmed with TrimGalore! Version version 4.0 parameters --length 20 --fastqc alignment with bwa mem version 0.7.15/ parameters -M or bowtie2 version 2.4.1/parameters --verysensitive conversion to bam and sorted and indexed with samtools version 1.3.1/ bigwigs made with DeepTools 3.3.1/ parameters --normalizeUsing CPM Deeptools for creation of heatmaps and metaplots Genome_build: GCA_000182925.2 (NC12) Supplementary_files_format_and_content: bigwig
|
|
|
Submission date |
Jul 15, 2020 |
Last update date |
Nov 14, 2020 |
Contact name |
Zachary A Lewis |
E-mail(s) |
zacharyaustinlewis@gmail.com
|
Organization name |
University of Georgia
|
Department |
Microbiology
|
Lab |
Lewis
|
Street address |
1000 Cedar St. Biosciences Bldg
|
City |
Athens |
State/province |
GA |
ZIP/Postal code |
30602 |
Country |
USA |
|
|
Platform ID |
GPL20660 |
Series (1) |
GSE154497 |
Chromatin accessibility profiling in Neurospora crassa reveals features associated with accessible and inaccessible chromatin |
|
Relations |
BioSample |
SAMN15544542 |
SRA |
SRX8737831 |