|
| Status |
Public on Jul 18, 2024 |
| Title |
S2.Myc.IKK.1 |
| Sample type |
RNA |
| |
|
| Source name |
FACS purified
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Myc.IKK2
|
| Treatment protocol |
B cells were stimulated with Lipopolysaccharide (LPS; Sigma-Aldrich) at a concentration of 10ug/mL.
|
| Growth protocol |
Cells were grown at 1x106/mL in B-cell media (DMEM high glucose with Glutamax (Gibco), supplemented with Fetal Bovine Serum (GE Healthcare), non-essential aminoacids (Gibco), HEPES buffer (Gibco), sodium pyruvate (Gibco), Penicillin-Streptomycin (Gibco), and 2-Mercaptoethanol (Sigma-Aldrich), at 37 °C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
AllPrep DNA/RNA Mini and Micro Kits (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Plasmablast
|
| Data processing |
Raw illumina text files were read into R(version3.4) and normalised using the lumi package
|
| |
|
| Submission date |
Jul 21, 2020 |
| Last update date |
Jul 18, 2024 |
| Contact name |
Dinis Calado |
| E-mail(s) |
dinis.calado@crick.ac.uk
|
| Organization name |
The Francis Crick Institute
|
| Street address |
Midland Road
|
| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE154814 |
Co-activation of NF-κB and MYC renders cancer cells addicted to IL6 for survival and phenotypic stability |
|
