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Sample GSM4698212 Query DataSets for GSM4698212
Status Public on Dec 31, 2021
Title WT_Day12_Rep2
Sample type SRA
 
Source name bone marrow mononuclear cells
Organism Homo sapiens
Characteristics donor: healthy control
timepoint: Day12
tissue: bone marrow mononuclear cells
Growth protocol IMDM, 2% human AB plasma, 3% human AB serum, 1x Pen/Strep, 3IU/ml heparin, 10µg/ml insulin, 200µg/ml holo-transferrin, 1 IU erythropoetin, 10ng/ml SCF, 1ng/ml IL-3.
Extracted molecule genomic DNA
Extraction protocol https://doi.org/10.17504/protocols.io.wvgfe3w
CUT&RUN targeting GATA1
 
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina NextSeq 500
 
Data processing Library strategy: CUT&RUN targeting GATA1
FastQ files were trimmed, aligned, and filtered using CUT&RUNTools version 20200104
Occupancy peaks were determined using macs2 as part of the CUT&RUNTools pipeline
Genome_build: hg19
Supplementary_files_format_and_content: Narrow peak files per replicate
 
Submission date Jul 28, 2020
Last update date Dec 31, 2021
Contact name Caleb Lareau
E-mail(s) lareauc@mskcc.org
Organization name Memorial Sloan Kettering
Street address 417 E 68th St, Zuckerman - ZRC 1132
City New York
State/province New York
ZIP/Postal code 10065
Country USA
 
Platform ID GPL18573
Series (2)
GSE133417 A Congenital Anemia Dissociates the Pleiotropic Functions of Master Transcription Factor GATA1
GSE155253 A Congenital Anemia Dissociates the Pleiotropic Functions of Master Transcription Factor GATA1 [Patient Cut&Run]
Relations
BioSample SAMN15659822
SRA SRX8838510

Supplementary file Size Download File type/resource
GSM4698212_CR_GATA1_Leif_WT_Day12_r2_S35_aligned_reads_peaks.narrowPeak.gz 888.5 Kb (ftp)(http) NARROWPEAK
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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