|
| Status |
Public on Apr 09, 2012 |
| Title |
RH server number 17 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
RH server number 17
|
| Organisms |
Homo sapiens; Cricetulus griseus |
| Characteristics |
cell line: G3 radiation hybrid
gender: male/male
|
| Growth protocol |
Cells were grown in alpha MEM with 10% FBS, 1x ampicillin and 1x HAT
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using the Qiagen DNeasy Tissue kit according to the manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
The samples were labeled using the Agilent Geomic DNA Labeling Kit PLUS (Agilent p/n 5188-5309) according to manufacturer's instructions. Genomic DNA was fragmented using restriction exzymes. Random hexamers were used to prime an Exo-Klenow fragment DNA polymerase reaction incorporating cy5/cy3 labeled dUTP.
|
| |
|
| Channel 2 |
| Source name |
A23 cell line
|
| Organism |
Cricetulus griseus |
| Characteristics |
gender: male
|
| Growth protocol |
Cells were grown in alpha MEM with 10% FBS, 1x ampicillin and 1x HAT
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using the Qiagen DNeasy Tissue kit according to the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
The samples were labeled using the Agilent Geomic DNA Labeling Kit PLUS (Agilent p/n 5188-5309) according to manufacturer's instructions. Genomic DNA was fragmented using restriction exzymes. Random hexamers were used to prime an Exo-Klenow fragment DNA polymerase reaction incorporating cy5/cy3 labeled dUTP.
|
| |
|
| |
| Hybridization protocol |
The samples were hybridized for 40 hours using the Agilent Oligo aCGH Hybridization Kit according to the manufacturer's instructions.
|
| Scan protocol |
Scanned on an Agilent G2505B scanner.
Images were quantified using Agilent Feature Extraction Software version 9.5.1.1
|
| Description |
US23502366_251469327169_S01_CGH-v4_95_Feb07.txt
|
| Data processing |
Data from each array was mean centered. The data across all arrays was aggregated and bimodal. The data was scaled so that the second mode was centered at log10(3/2) for autosomes and log10(2/1) for sex chromosomes.
|
| |
|
| Submission date |
Nov 12, 2009 |
| Last update date |
Apr 09, 2012 |
| Contact name |
Richard T Wang |
| Organization name |
UCLA
|
| Street address |
650 Charles E Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL9128 |
| Series (1) |
| GSE19003 |
Seeking novel mammalian genes using transcript profiling and genotyping of a human radiation hybrid panel |
|
