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Sample GSM4700907 Query DataSets for GSM4700907
Status Public on Jul 30, 2020
Title P3
Sample type SRA
 
Source name Splenic plasmablast
Organism Mus musculus
Characteristics strain: Balb/c
day post infection (dpi): 14
preganancy status: Pregnant
Extracted molecule total RNA
Extraction protocol 10,000 plasmablasts (CD138+B220int 229 ) were sorted from the spleens of pregnant and Pregnancy Confounds Influenza-Specific Humoral Responses Pregnancy Confounds Influenza-Specific Humoral Responses
Single-end RNA sequencing was performed on samples in duplicate to increase sequencing depth (101 base pair reads).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing The Rsubread package align function was used to align single end reads to the Mus musculus genome (GRCm38.93), BAM file created after alignment
Duplicate BAM files were merged together
Genes were counted using the featureCounts function of Rsubread
Genome_build: GRCm38.93
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample in cvs file
 
Submission date Jul 29, 2020
Last update date Jul 30, 2020
Contact name Lisa Mills
E-mail(s) lisa.k.mills87@gmail.com
Organization name CDC
Street address 1600 Clifton Road
City Atlanta
State/province GA
ZIP/Postal code 30021
Country USA
 
Platform ID GPL13112
Series (1)
GSE155388 Pregnancy Downregulates Plasmablast Metabolic Gene Expression Following Influenza without Altering Long-Term Antibody Function
Relations
BioSample SAMN15668990
SRA SRX8845606

Supplementary file Size Download File type/resource
GSM4700907_P3.txt.gz 225.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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