|
| Status |
Public on Feb 28, 2021 |
| Title |
HCC1806 negative control GapmeR N2 |
| Sample type |
RNA |
| |
|
| Source name |
HCC1806 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCC1806 cells
treatment: negative control GapmeR
|
| Treatment protocol |
cultured cells treated for 48hours with GapmeRs
|
| Growth protocol |
The cell lines were obtained from the American Type Culture Collection (ATCC) and were cultured as per ATCC guidelines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
cells were collected in TRIzol and total RNA purified using a PureLink RNA kit (Invitrogen Thermo Fisher Scientific) and incorporating a DNAse treatment step following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix WT Plus kit and biotin allonamide triphosphate
|
| |
|
| Hybridization protocol |
16h at 45oC, Affymetrix Human Gene 2.0 ST microarray platform
|
| Scan protocol |
Affymetrix genechip scanner 3000 (Scanner#4)
|
| Data processing |
RMA expression value derived from Expression Console software
|
| |
|
| Submission date |
Aug 12, 2020 |
| Last update date |
Mar 01, 2021 |
| Contact name |
Paola Marcato |
| E-mail(s) |
paola.marcato@dal.ca
|
| Phone |
9024944239
|
| Organization name |
Dalhousie University
|
| Department |
Pathology
|
| Street address |
11F11, 5850 College Street
|
| City |
Halifax |
| State/province |
Nova Scotia |
| ZIP/Postal code |
B3H 4R2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE156114 |
The effect of lncRNA PART1 knockdown on gene expression in HCC1806 and HCC1395 cells |
|
