|
| Status |
Public on Apr 01, 2021 |
| Title |
VPA 6M-4 |
| Sample type |
RNA |
| |
|
| Source name |
Cortical tissue of Area12 from VPA 6M marmoset
|
| Organism |
Callithrix jacchus |
| Characteristics |
animal id: 13090
Sex: F
treatment: VPA
age (days): 180
area: Area12
|
| Treatment protocol |
Marmosets were anesthesized with ketamine followed by pentobarbital. Diethylpyrocarbonate-treated phosphate-buffered saline was transcardially perfused. Cortical tissues were isolated and immersed in RNAlater (Thermo Fisher).
|
| Growth protocol |
For production of VPA marmoset, pregnant marmoset was administered with VPA at post-conception days 60-66, and the offspring were used.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was generated using the GeneChip 3'IVT Express Kit (Affymetrix) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The arrays were hybridized and washed using the GeneChip Hybridization, Wash, and Stain Kit (Affymetrix) according to the manufacturer's instructions.
|
| Scan protocol |
The arrays were scanned using the GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from VPA 6M
|
| Data processing |
The data were normalized by GCRMA.
|
| |
|
| Submission date |
Aug 13, 2020 |
| Last update date |
Apr 02, 2021 |
| Contact name |
Satoshi Watanabe |
| Organization name |
National Center of Neurology and Psychiatry
|
| Street address |
4-1-1 Ogawa Higashi
|
| City |
Kodaira |
| State/province |
Tokyo |
| ZIP/Postal code |
187-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL29019 |
| Series (1) |
| GSE156186 |
Expression data from early postnatal brain in autism model marmoset |
|
