|
| Status |
Public on Nov 26, 2009 |
| Title |
Total leucocytes |
| Sample type |
RNA |
| |
|
| Source name |
FACS sorted total leukocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Total leucocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the miRNeasy mini kit (Qiagen) according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Samples were spiked with RNA spike control oligos (Genisphere) and labeled according to the GenisphereÒ solution using the Genisphere FlashTag™ Biotin RNA Labeling Kit.
|
| |
|
| Hybridization protocol |
Biotin labeled samples were mixed with the eukaryotic hybridization controls (Affymetrix) and hybridized to Affymetrix miRNA arrays. Arrays were subsequently washed and stained in the GeneChip fluidics station 450 (Affymetrix).
|
| Scan protocol |
To assess the raw probe signal intensities, chips were scanned using the GeneChip scanner 3000 (Affymetrix).
|
| Description |
miRNA expression data of total leukocytes
|
| Data processing |
RMA normalization and probe summarization was carried out on the human specific and control probe sets only.
|
| |
|
| Submission date |
Nov 25, 2009 |
| Last update date |
Nov 25, 2009 |
| Contact name |
Rekin's Janky |
| E-mail(s) |
Nucleomics.Bioinformatics@vib.be
|
| Organization name |
VIB
|
| Department |
Nucleomics Core
|
| Street address |
Herestraat 49 Box 816
|
| City |
Leuven |
| ZIP/Postal code |
B-3000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE19183 |
miRNA profiling of FACS sorted human leukocyte cell types |
|
