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Sample GSM4756933 Query DataSets for GSM4756933
Status Public on Dec 07, 2020
Title PloomTECHplus-1
Sample type SRA
Source name Human Caucasian lung carcinoma
Organism Homo sapiens
Characteristics cell line: A549
cell type: Human Caucasian lung carcinoma cell line
treatment: PloomTECHplus
Treatment protocol Each samples were treated with 2percent DMSO control, 3R4F, PloomTECH,and Ploom TECH plus for 4hours.
Growth protocol A549 cells were grown in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10 µg/ml gentamicin and 10% fetal bovine serum (Thermo Fisher Scinetific).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using ISOGEN II reagent (NIPPON GENE), according to the manufacturer’s protocol.
RNA-seq libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
Description A549-PloomTECHplus2-1_S5
Data processing Basecalls performed using Illumina MiSeq
RNA-seq reads were aligned to the human hg38 genome assembly using Illumina MiSeq
RPKM were calculated using Partek Genomic suite version 6.6
Genome_build: human hg38
Supplementary_files_format_and_content: RPKM
Submission date Aug 31, 2020
Last update date Dec 07, 2020
Contact name Naoko Hattori
Organization name Nagasaki university
Street address Sakamoto 1-12-4
City Nagasaki
ZIP/Postal code 852-8523
Country Japan
Platform ID GPL15520
Series (1)
GSE157165 Cigarette smoke but not novel tobacco vapor product cause epigenetic disruption and cell apoptosis.
BioSample SAMN15946776
SRA SRX9040289

Supplementary file Size Download File type/resource
GSM4756933_PloomTECHplus-1-RPKM.txt.gz 163.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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