|
| Status |
Public on Dec 07, 2020 |
| Title |
PloomTECHplus-1 |
| Sample type |
SRA |
| |
|
| Source name |
Human Caucasian lung carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: Human Caucasian lung carcinoma cell line
treatment: PloomTECHplus
|
| Treatment protocol |
Each samples were treated with 2percent DMSO control, 3R4F, PloomTECH,and Ploom TECH plus for 4hours.
|
| Growth protocol |
A549 cells were grown in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10 µg/ml gentamicin and 10% fetal bovine serum (Thermo Fisher Scinetific).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using ISOGEN II reagent (NIPPON GENE), according to the manufacturer’s protocol.
RNA-seq libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
A549-PloomTECHplus2-1_S5
|
| Data processing |
Basecalls performed using Illumina MiSeq
RNA-seq reads were aligned to the human hg38 genome assembly using Illumina MiSeq
RPKM were calculated using Partek Genomic suite version 6.6
Genome_build: human hg38
Supplementary_files_format_and_content: RPKM
|
| |
|
| Submission date |
Aug 31, 2020 |
| Last update date |
Dec 07, 2020 |
| Contact name |
Naoko Hattori |
| E-mail(s) |
aa20111005@gmail.com
|
| Organization name |
Nagasaki university
|
| Street address |
Sakamoto 1-12-4
|
| City |
Nagasaki |
| ZIP/Postal code |
852-8523 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15520 |
| Series (1) |
| GSE157165 |
Cigarette smoke but not novel tobacco vapor product cause epigenetic disruption and cell apoptosis. |
|
| Relations |
| BioSample |
SAMN15946776 |
| SRA |
SRX9040289 |
