|
| Status |
Public on Aug 15, 2013 |
| Title |
S100P overexpressing clone 1, b |
| Sample type |
RNA |
| |
|
| Source name |
S100P overexpressing clone 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Panc1
cell type: pancreatic cancer
vector: S100P overexpressing
|
| Treatment protocol |
Cells were grown to 70-80% confluence before RNA extraction.
|
| Growth protocol |
Panc1 stable cell lines were maintained in DMEM with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using the Ambion RNAqueous kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000.
|
| Description |
PANC1_S1b
|
| Data processing |
Robust multi-array analysis (RMA) using R.
|
| |
|
| Submission date |
Dec 02, 2009 |
| Last update date |
Aug 16, 2013 |
| Contact name |
Claude Chelala |
| E-mail(s) |
c.chelala@qmul.ac.uk
|
| Organization name |
Barts & The London School of Medicine (QMUL)
|
| Department |
Institute of Cancer
|
| Lab |
Centre for Molecular Oncology & Imaging
|
| Street address |
Charterhouse square
|
| City |
London |
| State/province |
London |
| ZIP/Postal code |
EC1M6BQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE19278 |
S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells [HG-U133_Plus_2] |
| GSE19281 |
S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells |
|
