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Sample GSM478449 Query DataSets for GSM478449
Status Public on Aug 15, 2013
Title S100P overexpressing clone 1, b
Sample type RNA
Source name S100P overexpressing clone 1
Organism Homo sapiens
Characteristics cell line: Panc1
cell type: pancreatic cancer
vector: S100P overexpressing
Treatment protocol Cells were grown to 70-80% confluence before RNA extraction.
Growth protocol Panc1 stable cell lines were maintained in DMEM with 10% FBS.
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed using the Ambion RNAqueous kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using GeneChip Scanner 3000.
Description PANC1_S1b
Data processing Robust multi-array analysis (RMA) using R.
Submission date Dec 02, 2009
Last update date Aug 16, 2013
Contact name Claude Chelala
Organization name Barts & The London School of Medicine (QMUL)
Department Institute of Cancer
Lab Centre for Molecular Oncology & Imaging
Street address Charterhouse square
City London
State/province London
ZIP/Postal code EC1M6BQ
Country United Kingdom
Platform ID GPL570
Series (2)
GSE19278 S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells [HG-U133_Plus_2]
GSE19281 S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells

Data table header descriptions
VALUE RMA-normalized intensity value (log-scale)

Data table
1007_s_at 10.02716655
1053_at 7.707755447
117_at 5.164301114
121_at 7.150051032
1255_g_at 2.750631016
1294_at 4.684025084
1316_at 3.988393703
1320_at 4.766756071
1405_i_at 2.881342616
1431_at 3.04099908
1438_at 5.072236945
1487_at 7.557506817
1494_f_at 5.292033862
1552256_a_at 9.279306742
1552257_a_at 7.227070482
1552258_at 4.191524255
1552261_at 3.682723589
1552263_at 5.361400466
1552264_a_at 7.702997697
1552266_at 2.974555767

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.

Supplementary file Size Download File type/resource
GSM478449.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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