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Sample GSM478451 Query DataSets for GSM478451
Status Public on Aug 15, 2013
Title S100P overexpressing clone 5, b
Sample type RNA
 
Source name S100P overexpressing clone 5
Organism Homo sapiens
Characteristics cell line: Panc1
cell type: pancreatic cancer
vector: S100P overexpressing
Treatment protocol Cells were grown to 70-80% confluence before RNA extraction.
Growth protocol Panc1 stable cell lines were maintained in DMEM with 10% FBS.
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed using the Ambion RNAqueous kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using GeneChip Scanner 3000.
Description PANC1_S5b
Data processing Robust multi-array analysis (RMA) using R.
 
Submission date Dec 02, 2009
Last update date Aug 16, 2013
Contact name Claude Chelala
E-mail(s) c.chelala@qmul.ac.uk
Organization name Barts & The London School of Medicine (QMUL)
Department Institute of Cancer
Lab Centre for Molecular Oncology & Imaging
Street address Charterhouse square
City London
State/province London
ZIP/Postal code EC1M6BQ
Country United Kingdom
 
Platform ID GPL570
Series (2)
GSE19278 S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells [HG-U133_Plus_2]
GSE19281 S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells

Data table header descriptions
ID_REF
VALUE RMA-normalized intensity value (log-scale)

Data table
ID_REF VALUE
1007_s_at 10.1412644
1053_at 7.871142385
117_at 6.306121015
121_at 7.192754795
1255_g_at 2.864469281
1294_at 4.920480063
1316_at 3.954496183
1320_at 4.551633069
1405_i_at 3.038541058
1431_at 3.055114941
1438_at 5.138497264
1487_at 7.802997657
1494_f_at 5.31968789
1552256_a_at 9.627272522
1552257_a_at 7.368238206
1552258_at 3.896436106
1552261_at 4.175125284
1552263_at 5.561026273
1552264_a_at 7.550400847
1552266_at 2.983051834

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM478451.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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